The test procedure

The procuct

AllergyScreen™ and AlleisaScreen™ are semi-quantitative in-vitro systems for proving the presence of specific IgE antibodies in serum. They offer an efficient and fast test with up to 20 (AllergyScreen™) or 30 flexible allergens (AlleisaScreen™) in one single reaction.

Special allergens which are extracted from certificated raw material and tested for their allergic reactivity with in-house serum controls are bound to the surface of nitrocellulose membranes lying in a reaction trough.

The test principle

The patient's serum is pipetted into the reaction trough and incubated at room temperature. During this time, the allergen-specific IgE-antibodies react with the allergens and bind to the nitrocellulose membranes via the allergen. Non-bound material is removed by washing.

After this, an anti-human IgE antibody coupled with biotin is added and incubated at room temperature. This binds to the respective specific IgE in the test fields from the first incubation. Non-bound detector antibodies are removed by washing.

Next, streptavidin is added, which is conjugated with alkaline phosphatise, and incubated at room temperature. This binds to the biotin from the second incubation in the test fields and to the positive control. Non-bound streptavidin conjugate is removed by washing.

After adding the substrate and incubating at room temperature, a specific enzymatic color reaction of the alkaline phosphatase takes place which results in the formation of precipitates on the test strips.

The coloration is directly proportional to the specific antibody content in the serum sample.